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Hoefer semiphor semi-dry transfer unit
Semiphor Semi Dry Transfer Unit, supplied by Hoefer, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/semiphor+semi-dry+transfer+unit/semiphor+semidry+transfer+unit/pm38836410-193-17-21
Average 90 stars, based on 1 article reviews
semiphor semi-dry transfer unit - by Bioz Stars, 2026-10
90/100 stars

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Article Title: Differential promoter usage of infA in response to cold shock in Escherichia coli.
Article Snippet: RNA samples (20 lg) were electrophoresed on a 5% polyacrylamide gel containing 7 M urea, and blotted to Hybond N+ (Amersham Biosciences) with a Semiphor Semi-dry transfer unit (Hoefer) at 180 mA for 1 h. Hybridization was performed as previously described [18,19].

Article Title: Role of the sequence of the rne-dependent site in 3' processing of M1 RNA, the catalytic component of Escherichia coli RNase P.
Article Snippet: RNAs were electrophoresed in a 5% polyacrylamide gel containing 7 M urea, electrotransferred to a nylon membrane (Hybond N+; Amersham) with a Hoefer SemiPhor Semi-dry transfer unit, and then they were hybridized, as described [8].

Article Title: Connexin50, a gap junction protein of macrogliaP6n the mammalian retina and visual pathway.
Article Snippet: Reverse transcriptase-polymerase chain reaction (RT-PCR), Western blotting and immunocytochemistry were used to study the expression of gap junction proteins (connexins ; Cx) in the rat and rabbit retina.. RT-PCR of rabbit total retinal RNA using primers selected for the human Cx50 (α8 Cx) DNA template yielded cDNA fragments of the predicted base pair size.. Western blots of rat and rabbit retinal membrane preparations probed with a monoclonal antibody which recognizes Cx50 in the lens of several mammalian species revealed a single band (MW 50 kD), identical to that recognized in lens membrane extracts.

Article Title: Oxygen-resistant [FeFe]hydrogenases: new biocatalysis tools for clean energy and cascade reactions.
Article Snippet: Proteins separated by SDS-PAGE were electro-transferred onto a PVDF Transfer Membrane 0.45 mm (Thermo Scientic) with a Semiphor semi-dry transfer unit (Hoefer).

Article Title: Antitumor activity of F90, an epidermal growth factor receptor tyrosine kinase inhibitor, on glioblastoma cell line SHG-44
Article Snippet: Equal amounts of protein were separated on 8% or 10% SDS-polyacrylamide gel by electrophoresis and blotted onto polyvinylidene fluoride membrane using the SemiPhor Semi-Dry transfer unit (Hoefer Scientific Instruments, San Francisco, CA, USA).

Article Title: An apparent association between glycosylphosphatidylinositol-anchored proteins and a sphingolipid in Tetrahymena mimbres
Article Snippet: For immunoblotting, the proteins were transferred to nitrocellulose membranes with a SemiPhor Semi-Dry transfer unit (Hoefer Scientific Instruments, San Francisco, CA, U.S.A.).

Article Title: An ELISA for antibodies to infectious bronchitis virus based on nucleocapsid protein produced in Escherichia coli
Article Snippet: The gel was transferred to a nitrocellulose (NC) membrane using Hoefer SemiPhor semi-dry transfer unit for 1.5 hrs according to the operation manual (Amersham Pharmacia Biotech Inc., NJ, USA).

Article Title: Expression of the soluble extracellular domain of human thrombopoietin receptor using a maltose-binding protein-affinity fusion system.
Article Snippet: Protein was transferred to PVDF membrane using a Hoefer SemiPhor semi-dry transfer unit (Pharmacia, Uppsala, Sweden).

Membrane:

Article Title: Differential promoter usage of infA in response to cold shock in Escherichia coli.
Article Snippet: RNA samples (20 lg) were electrophoresed on a 5% polyacrylamide gel containing 7 M urea, and blotted to Hybond N+ (Amersham Biosciences) with a Semiphor Semi-dry transfer unit (Hoefer) at 180 mA for 1 h. Hybridization was performed as previously described [18,19].

Article Title: Role of the sequence of the rne-dependent site in 3' processing of M1 RNA, the catalytic component of Escherichia coli RNase P.
Article Snippet: RNAs were electrophoresed in a 5% polyacrylamide gel containing 7 M urea, electrotransferred to a nylon membrane (Hybond N+; Amersham) with a Hoefer SemiPhor Semi-dry transfer unit, and then they were hybridized, as described [8].

Article Title: Connexin50, a gap junction protein of macrogliaP6n the mammalian retina and visual pathway.
Article Snippet: Reverse transcriptase-polymerase chain reaction (RT-PCR), Western blotting and immunocytochemistry were used to study the expression of gap junction proteins (connexins ; Cx) in the rat and rabbit retina.. RT-PCR of rabbit total retinal RNA using primers selected for the human Cx50 (α8 Cx) DNA template yielded cDNA fragments of the predicted base pair size.. Western blots of rat and rabbit retinal membrane preparations probed with a monoclonal antibody which recognizes Cx50 in the lens of several mammalian species revealed a single band (MW 50 kD), identical to that recognized in lens membrane extracts.

Article Title: Oxygen-resistant [FeFe]hydrogenases: new biocatalysis tools for clean energy and cascade reactions.
Article Snippet: Proteins separated by SDS-PAGE were electro-transferred onto a PVDF Transfer Membrane 0.45 mm (Thermo Scientic) with a Semiphor semi-dry transfer unit (Hoefer).

Article Title: Antitumor activity of F90, an epidermal growth factor receptor tyrosine kinase inhibitor, on glioblastoma cell line SHG-44
Article Snippet: Equal amounts of protein were separated on 8% or 10% SDS-polyacrylamide gel by electrophoresis and blotted onto polyvinylidene fluoride membrane using the SemiPhor Semi-Dry transfer unit (Hoefer Scientific Instruments, San Francisco, CA, USA).

Article Title: An apparent association between glycosylphosphatidylinositol-anchored proteins and a sphingolipid in Tetrahymena mimbres
Article Snippet: For immunoblotting, the proteins were transferred to nitrocellulose membranes with a SemiPhor Semi-Dry transfer unit (Hoefer Scientific Instruments, San Francisco, CA, U.S.A.).

Article Title: An ELISA for antibodies to infectious bronchitis virus based on nucleocapsid protein produced in Escherichia coli
Article Snippet: The gel was transferred to a nitrocellulose (NC) membrane using Hoefer SemiPhor semi-dry transfer unit for 1.5 hrs according to the operation manual (Amersham Pharmacia Biotech Inc., NJ, USA).

Article Title: Expression of the soluble extracellular domain of human thrombopoietin receptor using a maltose-binding protein-affinity fusion system.
Article Snippet: Protein was transferred to PVDF membrane using a Hoefer SemiPhor semi-dry transfer unit (Pharmacia, Uppsala, Sweden).

Nucleic Acid Electrophoresis:

Article Title: Differential promoter usage of infA in response to cold shock in Escherichia coli.
Article Snippet: RNA samples (20 lg) were electrophoresed on a 5% polyacrylamide gel containing 7 M urea, and blotted to Hybond N+ (Amersham Biosciences) with a Semiphor Semi-dry transfer unit (Hoefer) at 180 mA for 1 h. Hybridization was performed as previously described [18,19].

Article Title: Role of the sequence of the rne-dependent site in 3' processing of M1 RNA, the catalytic component of Escherichia coli RNase P.
Article Snippet: RNAs were electrophoresed in a 5% polyacrylamide gel containing 7 M urea, electrotransferred to a nylon membrane (Hybond N+; Amersham) with a Hoefer SemiPhor Semi-dry transfer unit, and then they were hybridized, as described [8].

Article Title: Connexin50, a gap junction protein of macrogliaP6n the mammalian retina and visual pathway.
Article Snippet: Reverse transcriptase-polymerase chain reaction (RT-PCR), Western blotting and immunocytochemistry were used to study the expression of gap junction proteins (connexins ; Cx) in the rat and rabbit retina.. RT-PCR of rabbit total retinal RNA using primers selected for the human Cx50 (α8 Cx) DNA template yielded cDNA fragments of the predicted base pair size.. Western blots of rat and rabbit retinal membrane preparations probed with a monoclonal antibody which recognizes Cx50 in the lens of several mammalian species revealed a single band (MW 50 kD), identical to that recognized in lens membrane extracts.

Article Title: Oxygen-resistant [FeFe]hydrogenases: new biocatalysis tools for clean energy and cascade reactions.
Article Snippet: Proteins separated by SDS-PAGE were electro-transferred onto a PVDF Transfer Membrane 0.45 mm (Thermo Scientic) with a Semiphor semi-dry transfer unit (Hoefer).

Article Title: Antitumor activity of F90, an epidermal growth factor receptor tyrosine kinase inhibitor, on glioblastoma cell line SHG-44
Article Snippet: Equal amounts of protein were separated on 8% or 10% SDS-polyacrylamide gel by electrophoresis and blotted onto polyvinylidene fluoride membrane using the SemiPhor Semi-Dry transfer unit (Hoefer Scientific Instruments, San Francisco, CA, USA).

Article Title: An apparent association between glycosylphosphatidylinositol-anchored proteins and a sphingolipid in Tetrahymena mimbres
Article Snippet: For immunoblotting, the proteins were transferred to nitrocellulose membranes with a SemiPhor Semi-Dry transfer unit (Hoefer Scientific Instruments, San Francisco, CA, U.S.A.).

Article Title: An ELISA for antibodies to infectious bronchitis virus based on nucleocapsid protein produced in Escherichia coli
Article Snippet: The gel was transferred to a nitrocellulose (NC) membrane using Hoefer SemiPhor semi-dry transfer unit for 1.5 hrs according to the operation manual (Amersham Pharmacia Biotech Inc., NJ, USA).

Article Title: Expression of the soluble extracellular domain of human thrombopoietin receptor using a maltose-binding protein-affinity fusion system.
Article Snippet: Protein was transferred to PVDF membrane using a Hoefer SemiPhor semi-dry transfer unit (Pharmacia, Uppsala, Sweden).



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